Direct determination of plasma endothelin-I by chemiluminescence enzyme immunoassay.

نویسندگان

  • R Iwata
  • T Hayashi
  • Y Nakao
  • M Yamaki
  • T Yoshimasa
  • H Ito
  • Y Saito
  • M Mukoyam
  • K Nakao
چکیده

A highly sensitive sandwich-type chemiluminescence enzyme immunoassay for plasma endothelin-I (ET-1), involving no extraction steps, has been developed. Two populations of polyclonal antibodies were used in the present study: One is specific to the C-terminus of the endothelin family of peptides; the other, a Fab' fragment against the N-terminal core region of ET-1, is coupled with horseradish peroxidase (HRP). Labeled HRP activity was measured by using an enhanced chemiluminescence reaction of luminol/hydrogen peroxide. The assay was sensitive enough to detect 0.5 ng/L (0.05 pg/well) of plasma ET-1 and had no significant cross-reactivities with other related peptides, including endothelin-3 and the endothelin precursor peptide, big ET-1. Reliability of the assay was confirmed by comparison with an extraction-based procedure and a commercial kit.

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عنوان ژورنال:
  • Clinical chemistry

دوره 42 8 Pt 1  شماره 

صفحات  -

تاریخ انتشار 1996